A simple, efficient, precise and accurate spectroscopic method has been developed and validated for quantitative estimation of embelin in bulk and churna formulation. Embelin standard solution was scanned in the UV range (400-200 nm) in a 1 cm quartz cell in a double beam UV spectrophotometer. The max of embelin was 303 nm. The method obeys beers law in the concentration range from 5-17.5 μg/ml. The correlation coefficient was found to be 0.999 and regression of the curve was found y = 0.036x - 0.056 with excellent recovery 99-101%. Limit of detection and limit of quantitation were found to be 2.65 μg/ml and 4.03 μg/ml respectively. The method was validated for several parameters like accuracy, precision as per ICH guidelines. Value of % RSD and % recovery was found satisfactory, hence the proposed method is precise, accurate and economical hence can be used for routine analysis.
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